Fully automated whole blood CyTOF phospho flow stimulation and staining

Whole blood CyTOF phospho flow stimulation and staining.

Abbreviated video leaves out a lot of the staining and wash steps.

Starts with aliquoting cytokines and other stimuli, then adding cytokines to blood (0:34), going into the incubator (1:02), fixing (1:17), lysing RBCs (1:37), washing (1:50), multiplexing through "barcoding" (2:20), rearranging tips at 2:38, combining cells together at 2:50, washing (3:03), … and done!

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