Total Dietary Fiber Video Method (AOAC Method 991.43/AACC method 32-07.01) with K-TDFR

Our scientists demonstrate the full assay procedure of Dietary Fiber (AOAC Method 991.43 / AACC method 32-07.01) using Megazyme’s Total Dietary Fiber Assay Kit, (K-TDFR)

** Subtitles are available, but must be manually turned on and can be changed to your desired language **

This Assay Kit can be found on our website: https://www.megazyme.com/total-dietary-fiber-assay-kit

00:06 Introduction
02:31 Principle
04:27 Preparation of Fritted Crucibles
04:44 Sample Preparation
05:43 Reagent Preparation
06:12 Weighing of Samples
06:35 Incubation with heat stable α-amylase
08:24 Incubation with Protease
09:35 Incubation with Amyloglucosidase
10:18 Method A – Measurement of TDF as HMWDF
14:44 Method B – Separation of TDF components into IDF and SDFP
14:48 Measurement of IDF
18:01 Precipitation & Recovery of SDFP component
19:19 Calculations

Download the K-TDFR assay protocol: https://support.megazyme.com/support/solutions/articles/8000025116-total-dietary-fiber-k-tdfr-data-booklet
Access the Mega-Calc for K-TDFR: https://support.megazyme.com/support/solutions/articles/8000025440-total-dietary-fiber-k-tdfr-mega-calc
Supporting Video: Preparation of crucibles with Celite Filter Beds https://youtu.be/v7UPAGZmGTw

AOAC 991.43TDFAACC 32-07.01